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  • Title: Isothermal Titration Calorimetry Measurements of Riboswitch-Ligand Interactions.
    Author: Jones CP, Piszczek G, Ferré-D'Amaré AR.
    Journal: Methods Mol Biol; 2019; 1964():75-87. PubMed ID: 30929236.
    Abstract:
    One of the many ways by which bacteria control gene expression is through cis-acting regulatory mRNA elements called riboswitches. By specifically binding to small molecules or metabolites and pairing the binding event to an RNA structure change, riboswitches link a metabolic input to a transcriptional or translational output. For over a decade, isothermal titration calorimetry (ITC) has been used to investigate how riboswitches interact with small molecules. We present methods for assaying RNA-ligand interactions using ITC and analyzing resulting data to estimate thermodynamic parameters associated with binding.
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