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  • Title: Enzyme Kinetics Determined by Single-Injection Isothermal Titration Calorimetry.
    Author: Quinn CF, Hansen LD.
    Journal: Methods Mol Biol; 2019; 1964():241-249. PubMed ID: 30929247.
    Abstract:
    This chapter describes how to collect Michaelis-Menten kinetic data on an enzyme-catalyzed reaction with the isothermal titration calorimetry (ITC) and the single-injection method. ITC measures the heat rate which is directly proportional to the reaction rate. The enthalpy change (ΔrH), Km, kcat, and vmax are determined in a single assay that does not require labeling or immobilization.
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