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  • Title: [Isolation by binding to ultrafiltration membranes of a testicular factor limiting the number of germ cells in fetal ovary of rats in vitro].
    Author: Prépin J, Charpentier G, Hida N, Jost A, Maingourd M.
    Journal: C R Acad Sci III; 1986; 303(4):123-6. PubMed ID: 3093011.
    Abstract:
    The medium used for culturing in vitro fetal or neonatal testes, when used subsequently to culture for 4 days ovaries from 13.5 day old rat fetuses, has the property of severely limiting the number of ovarian germ cells. The non-dialysable factor(s) responsible for the observed effect binds to ultrafiltration membranes (Diaflo, Amicon) and can be eluted from these membranes with fresh medium added 1 M NaCl.
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