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  • Title: Preliminary characterisation of torovirus-like particles of humans: comparison with Berne virus of horses and Breda virus of calves.
    Author: Beards GM, Brown DW, Green J, Flewett TH.
    Journal: J Med Virol; 1986 Sep; 20(1):67-78. PubMed ID: 3093635.
    Abstract:
    Pleomorphic virus-like particles have been observed by electron microscopy in the faeces of children and adults with diarrhoea. Some of these particles were approximately 100 nm in diameter and had a "fringe" of closely applied peplomers approximately 10 nm long; they closely resembled Berne virus of horses and Breda virus of calves, the two representatives of a newly proposed family called the Toroviridae. In one sample a toroidal nucleoprotein-like structure was observed within the particles. For two samples a buoyant density of 1.14 g/ml was determined by centrifugation through a sucrose density gradient. One sample possessed a haemagglutinin for rat erythrocytes. The serological relationship between these different viruses was observed by immune electron microscopy, haemagglutination inhibition, and serum neutralisation. The role of these virus-like particles as candidate pathogens of humans is discussed.
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