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  • Title: Detection of TRH extended peptides in rat hypothalamus using an antibody raised to pGluHisProGlyLys.
    Author: Cockle SM, Smyth DG.
    Journal: Biosci Rep; 1986 Jun; 6(6):519-26. PubMed ID: 3094600.
    Abstract:
    An antibody was raised to the synthetic pentapeptide pGluHisProGlyLys which, in radioimmunoassay (RIA), could detect the pentapeptide at a level of 10 fmole per tube and exhibited less than 0.5 per cent cross reactivity with a series of related peptides. The RIA was used to demonstrate the presence of C-terminally extended forms of thyrotropin releasing hormone (TRH) in rat hypothalamus. After extraction, the endogenous peptides were resolved by gel exclusion chromatography and TRH-extended peptides were revealed by trypsin digestion to release the pentapeptide. The TRH extended peptides occurred in substantial quantity, approximately 11 pmoles/g, indicating that only partial processing of the gene duplicated prohormone takes place.
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