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  • Title: Pre-freezing Treatment with Butylated Hydroxytoluene and Cholesterol-loaded Methyl-β-cyclodextrin Improves Quality of Cryopreserved Boar Semen.
    Author: Baishya SK, Biswas RK, Govindasamy K, Deka BC, Sinha S, Singh M.
    Journal: Cryo Letters; 2018; 39(5):336-344. PubMed ID: 30963166.
    Abstract:
    BACKGROUND: Pre-freezing treatment of boar sperm with additives improves the quality of post-thaw sperms. OBJECTIVES: The study aimed to determine the efficacy of butylated hydroxy-toluene (BHT) and cholesterol-loaded methyl-β-cyclodextrin (CLC) for the improvement of the frozen-thawed boar sperm quality. METHODS: Split samples of 30 ejaculates from six boars were cryopreserved in lactose-egg yolk-glycerol extender containing BHT (0.2 mM), CLC (5 mg/ 200-240 x 106 sperm) or BHT (0.2 mM) plus CLC (5 mg per 200-240 x 106 sperm). Semen samples were evaluated for motility, membrane integrity, acrosomal status, mitochondrial membrane potential (MMP), DNA integrity and lipid peroxidation after equilibration and after freezing. RESULTS: The addition of BHT and CLC into the extender significantly improved (P<0.05) plasma membrane integrity and decreased (P<0.05) lipid peroxidation after freezing. Post-thaw motility and live intact acrosome were significantly (P<0.05) higher in the extenders with BHT or BHT plus CLC. The post-thaw MMP of viable spermatozoa and DNA integrity were not affected. BHT plus CLC showed a significant (P<0.05) improvement on motility as compared to BHT and CLC alone. CONCLUSION: Treatment of boar spermatozoa with BHT and CLC improved post-thaw sperm quality.
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