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  • Title: Blebbing, free Ca2+ and mitochondrial membrane potential preceding cell death in hepatocytes.
    Author: Lemasters JJ, DiGuiseppi J, Nieminen AL, Herman B.
    Journal: Nature; ; 325(6099):78-81. PubMed ID: 3099216.
    Abstract:
    Cell surface 'blebbing' is an early consequence of hypoxic and toxic injury to cells. A rise in cytosolic free Ca2+ has been suggested as the stimulus for bleb formation and the final common pathway to irreversible cell injury. Here, using digitized low-light video microscopy, we examine blebbing, cytosolic free Ca2+, mitochondrial membrane potential and loss of cell viability in individual cultured hepatocytes. Unexpectedly, we found that after 'chemical hypoxia' with cyanide and iodoacetate, cytosolic free Ca2+ does not change during bleb formation or before loss of cellular viability. Cell death was precipitated by a sudden breakdown of the plasma membrane permeability barrier, possibly caused by rupture of a cell surface bleb.
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