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  • Title: Biosynthesis regulation of the beta-glucosidase produced by a yeast strain transformed by genetic engineering.
    Author: Leclerc M, Chemardin P, Arnaud A, Ratomahenina R, Galzy P, Gerbaud C, Raynal A.
    Journal: Arch Microbiol; 1986 Nov; 146(2):115-7. PubMed ID: 3099720.
    Abstract:
    The biosynthesis of the beta-glucosidase enzyme was studied in a transformed yeast obtained by cloning in Saccharomyces cerevisiae the structural gene coding for beta-glucosidase in Kluyveromyces fragilis. The enzyme biosynthesis was found to be non-adaptative, and repressed by glucose. These features are similar to those observed in K. fragilis. beta-Glucosidase activity in the transformed yeast was much higher than in K. fragilis. We attempted to ferment cellobiose with the transformed yeast: practically no cellobiose was consumed, growth and ethanol production were negligible. Warburg experiments showed that cellobiose fermentation did not occur when the respiratory chain was not functioning.
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