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  • Title: Quantitative determination of human plasma apolipoprotein A-II by a non competitive enzyme-linked immunosorbent assay.
    Author: Puchois P, Kandoussi A, Duriez P, Fruchart JC, McConathy WJ, Koren E.
    Journal: J Immunoassay; 1986; 7(4):285-307. PubMed ID: 3100579.
    Abstract:
    A noncompetitive enzyme-linked immunosorbent assay (ELISA) for apolipoprotein A-II (ApoA-II) was developed. Microtiter plates were coated with affinity purified antibodies to ApoA-II. After incubation with human plasma, the amount of ApoA-II bound to the coated plate was determined with peroxidase-labeled antibodies to ApoA-II. When pure ApoA-II or delipidated reference plasma was used as standard, a single step delipidation was required in order to unmask some antigenic sites of ApoA-II. However, the underestimated ApoA-II values in untreated samples were shown to be corrected by using intact reference plasma as secondary standard. The average concentration of ApoA-II in normolipidemic plasma was 0.376 g/l.
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