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  • Title: Cloning and transcriptional regulation of the elastase lasA gene in mucoid and nonmucoid Pseudomonas aeruginosa.
    Author: Goldberg JB, Ohman DE.
    Journal: J Bacteriol; 1987 Mar; 169(3):1349-51. PubMed ID: 3102460.
    Abstract:
    The lasA gene (whose product is involved in the production of extracellular elastolytic activity) was isolated from a genomic bank containing DNA from Pseudomonas aeruginosa FRD1. Recombinant plasmid pELA1, containing the lasA gene, complemented the temperature-sensitive elastase mutation (lasA1) in P. aeruginosa PAO-E64. The lasA gene was physically mapped on plasmid pELA1 by deletion analysis and transposon mutagenesis. The direction of transcription of lasA was determined with a promoterless chloramphenicol acetyltransferase cartridge. The lasA-chloramphenicol acetyltransferase plasmid was transferred to isogenic mucoid and nonmucoid strains of P. aeruginosa FRD; the transcription of lasA-chloramphenicol acetyltransferase was slightly higher in the nonmucoid strain.
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