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  • Title: Identification of the calmodulin-binding components in canine cardiac sarcolemma.
    Author: Louis CF, Jarvis B, Turnquist J.
    Journal: Cell Calcium; 1987 Feb; 8(1):43-52. PubMed ID: 3103924.
    Abstract:
    The covalent attachment of 125I-calmodulin to canine cardiac sarcolemma has been achieved using the crosslinker dithiobis(succinimidyl propionate). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the crosslinked products revealed three 125I-calmodulin-labeled components of Mr = 125,000, 108,000 and 81,000. That the formation of these three components was Ca-dependent and inhibited by unlabeled calmodulin, or calmodulin antagonists, would indicate that the formation of these components was calmodulin-specific. The size of these 125I-labeled components was unchanged over a range of crosslinker or 125I-calmodulin concentrations indicating that they represent 1:1 complexes between 125I-calmodulin (Mr = 17,000) and Mr = 108,000, 91,000 and 64,000 sarcolemma components respectively. The labeling of these components with 125I-calmodulin was not enhanced when endogenous calmodulin was removed from sarcolemma. The possible identity of the 125I-calmodulin-labeled sarcolemma components is discussed.
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