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  • Title: Study on the wall-breaking method of carotenoids producing yeast Sporidiobolus pararoseus and the antioxidant effect of four carotenoids on SK-HEP-1 cells.
    Author: Liu C, Cheng Y, Du C, Lv T, Guo Y, Han M, Pi F, Zhang W, Qian H.
    Journal: Prep Biochem Biotechnol; 2019; 49(8):767-774. PubMed ID: 31050593.
    Abstract:
    The cell wall of carotenoids producing yeast Sporidiobolus pararoseus was broken through five different methods: acid-heating method, dimethyl sulfoxide (DMSO) method, enzymatic method, high-pressure homogenization (HPH) method, and cell autolysis method. HPH method not only brought the optimum breaking effect (wall-breaking extent of 72.3%) and the highest carotenoid extraction rate (67.2%), but also had the advantages of short-time, simple process, safe, and pollution-free. After optimization, the wall-breaking extent and the carotenoid extraction rate were enhanced to 78.3% and 82.5%, respectively. And the optimum conditions of HPH were obtained as homogenization pressure 80 MPa, bacterial liquid concentration 8% and homogenization for three times. Moreover, cell experiments demonstrated that all of the four carotenoids (β-carotene, γ-carotene, torulene, and torularhodin) purified from intracellular products of S. pararoseus. had the effect of resistance to oxidative damage from hydrogen peroxide on SK-HEP-1 cells, and torulene showed the most notable effect among them.
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