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  • Title: Transfer of D-galactosyl groups to 6-O-substituted 2-acetamido-2-deoxy-D-glucose residues by use of bovine D-galactosyltransferase.
    Author: Palcic MM, Srivastava OP, Hindsgaul O.
    Journal: Carbohydr Res; 1987 Feb 01; 159(2):315-24. PubMed ID: 3105882.
    Abstract:
    Bovine D-galactosyltransferase was found to transfer D-galactose from UDP-galactose to 6-O-substituted 2-acetamido-2-deoxy-beta-D-glucopyranosides. The resulting 6-O-substituted N-acetyllactosamines were readily synthesized in milligram amounts and conveniently isolated on a reverse-phase support when prepared as the 8-methoxycarbonyloctyl glycosides. The 6-O-substitution tolerated by the enzyme include an alpha-L-fucopyranosyl group and the methyl ester of alpha-linked N-acetylneuraminic acid, but not the free acid itself. The product trisaccharides were characterized by 1H-n.m.r. spectroscopy and fast-atom-bombardment mass spectrometry.
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