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  • Title: Fusion of pro region of subtilisin to staphylococcal protein A and its secretion by Bacillus subtilis.
    Author: Vasantha N, Thompson LD.
    Journal: Gene; 1986; 49(1):23-8. PubMed ID: 3106154.
    Abstract:
    Subtilisin is synthesized as a preproenzyme in Bacillus subtilis. We fused that region of the subtilisin gene, (apr[BamP]), which encodes the signal sequence and pro region, to the mature gene sequence (spa) for a heterologous protein (staphylococcal protein A). B. subtilis cells harboring this gene fusion synthesized a fusion protein consisting of the signal and pro sequence of subtilisin fused to the protein A; the signal sequence was processed and a fusion protein (pro + protein A) was secreted into the growth medium.
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