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  • Title: Unresponsiveness of phospholipase C to the regulatory proteins Ns and Ni in pancreatic islets.
    Author: Blachier F, Segura MC, Malaisse WJ.
    Journal: Res Commun Chem Pathol Pharmacol; 1987 Mar; 55(3):335-55. PubMed ID: 3107094.
    Abstract:
    The possible participation of the regulatory proteins Ns and Ni in the regulation of phospholipase C activity in rat pancreatic islets was investigated. The islets were preincubated for 120 min with myo-[2-3H]inositol and the fractional outflow rate of [3H]inositol or production of [3H]inositol 1-phosphate was then measured. Glucagon failed to affect these metabolic variables, whether in the absence or presence of D-glucose. Pretreatment of the islets with cholera toxin also failed to affect basal or glucose-stimulated [3H]inositol outflow. Likewise, clonidine, which abolished insulin release evoked by D-glucose and carbamylcholine, failed to prevent the stimulant action of these secretagogues upon either [3H]inositol outflow or [3H]inositol 1-phosphate production. It is concluded that the regulatory proteins Ns and Ni apparently do not play any major role in the regulation of phosphoinositide phosphodiesterase activity in islet cells.
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