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  • Title: Characterization of chromosome and plasmid transformation in Bacillus subtilis using gently lysed protoplasts.
    Author: Akamatsu T, Sekiguchi J.
    Journal: Arch Microbiol; 1987 Jan; 146(4):353-7. PubMed ID: 3107511.
    Abstract:
    Competent cells of Bacillus subtilis were transformed with DNA from gently lysed protoplasts. Significant linkages among markers separated by distances of approximately 2.3% of the total chromosome were found, which have not been detected for conventional transformation. In comparison to previous reports, enhanced plasmid transformation was observed [4.0 X 10(7) transformants per microgram DNA (one transformant per 5 X 10(4) molecules added)], when competent cells were transformed with DNA from lysed protoplasts harboring pUB110.
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