These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Ultrathin films of a metal-organic framework prepared from 2-methylimidazole, manganese(II) and cobalt(II) with strong oxidase-mimicking activity for colorimetric determination of glutathione and glutathione reductase activity.
    Author: Zhang Y, Dai C, Liu W, Wang Y, Ding F, Zou P, Wang X, Zhao Q, Rao H.
    Journal: Mikrochim Acta; 2019 May 10; 186(6):340. PubMed ID: 31076874.
    Abstract:
    Nanosheets (NSs; type ZIF-67) of a metal organic framework (MOF) that was prepared from 2-methylimidazole, manganese(II) and cobalt(II) were obtained by an ultrasonic hydrothermal method. Their Mn(II) doping reached as much as 11.3%. The NSs inherit high porosity, a large specific surface, and a large number of active sites. They display superior oxidase-mimicking activity and can catalyze the oxidation of tetramethylbenzidine (TMB) by molecular oxygen to form blue oxTMB. Glutathione (GSH) can reduce oxTMB, so that less blue oxTMB will be present. A simple and rapid method was established for the colorimetric determination of GSH and of the activity of GSH reductase (GR), best at a wavelength of 652 nm. The response to GSH drops linearly in the 0.1-25 μM concentration range. The activity of GR can be quantified in the 0.1 - 3 mU⋅mL-1 activity range. The respective detection limits are 0.07 μM and 0.18 mU⋅mL-1. Graphical abstract Schematic presentation of colorimetric detection of glutathione and glutathione reductase activity by the oxidase-mimicking activity of Mn-Co nanosheets in a metal organic framework.
    [Abstract] [Full Text] [Related] [New Search]