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  • Title: Microsomal arachidonate bioconversion in rat small intestine during maturation.
    Author: Wu-Wang CY, Neu J.
    Journal: Prostaglandins Leukot Med; 1987 Apr; 27(1):71-80. PubMed ID: 3108906.
    Abstract:
    This study was designed to compare prostaglandin (PG)-synthesizing activity in rat small intestinal microsomes in the 1st, 3rd, and 6th weeks of life and at maturity (greater than 100 days). When an incubation system was used containing 2 mg microsomal protein, 0.5 mM (-)-epinephrine and 1 mM reduced glutathione, the highest PG-synthesizing activity was achieved by incubating 0.157 mM 1-(14)C-arachidonate (specific activity 2.6 X 10(6) dpm/mumol) at 37 degrees C for 5 min. The labeled metabolites were extracted and then separated with high performance liquid chromatography. The four PGs analyzed were 6-keto-prostaglandin F1 alpha (6-keto-PGF1 alpha), thromboxane B2, prostaglandin F2 alpha and prostaglandin E2. Enzymatic activity for the synthesis of 6-keto-PGF1 alpha was much higher than that for the other PGs. A significant difference was observed for the bioconversion from arachidonate to 6-keto-PGF1 alpha and total PGs among the four age groups of rats. The postweanling groups (week 6 and adult) showed significantly higher enzymatic activities for the syntheses of 6-keto-PGF1 alpha and total PGs than did the preweanling groups (weeks 1 and 3).
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