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  • Title: Rapid Detection of Exosomal MicroRNAs Using Virus-Mimicking Fusogenic Vesicles.
    Author: Gao X, Li S, Ding F, Fan H, Shi L, Zhu L, Li J, Feng J, Zhu X, Zhang C.
    Journal: Angew Chem Int Ed Engl; 2019 Jun 24; 58(26):8719-8723. PubMed ID: 31095853.
    Abstract:
    Exosomal microRNAs (miRNAs) are important biomarkers for clinical diagnosis and disease treatment monitoring. However, most approaches for exosomal miRNA detection are time-consuming, laborious, and expensive. Herein, we report a virus-mimicking fusogenic vesicle (Vir-FV) that enables rapid, efficient, and high-throughput detection of exosomal miRNAs within 2 h. Fusogenic proteins on Vir-FVs can specifically target the sialic-acid-containing receptors on exosomes, inducing efficient fusion of Vir-FVs and exosomes. Upon vesicle content mixing, the molecular beacons encapsulated in Vir-FVs specifically hybridize with the target miRNAs in the exosomes, generating fluorescence. Combined with flow cytometry, the Vir-FVs can not only detect exosomal miRNAs but also distinguish tumor exosomes from normal exosomes by sensing the tumor-related miRNAs, paving the way towards the rapid and efficient detection of exosomal miRNAs for diagnosis and prognosis prediction of diseases.
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