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  • Title: Intracellular phospholipase activity in rat heart: comparison between endogenous and exogenous substrates.
    Author: Termine E, Léonardi J, Lafont H, Nalbone G.
    Journal: Biochimie; 1987 Mar; 69(3):245-8. PubMed ID: 3111550.
    Abstract:
    Two methods were used to estimate the intracellular phospholipase activity in rat heart: one using exogenous radioactive substrate dispersed as unilamellar vesicles; the other using endogenous membrane hydrolysis and subsequent phospholipid and lysophospholipid separation by high-performance liquid chromatography and quantification by phosphorus determination. We found that the endogenous method provided a higher hydrolysis rate than the exogenous method and that phosphatidyl ethanolamine was a better substrate than phosphatidyl choline.
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