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  • Title: Allelic exclusion frequency analysis and molecular characteristics of immunoglobulins secreted by the hybridomas expressing both allelic genes.
    Author: Cherepakhin VV, Jackubov LZ, Ibraghimov AR, Rokhlin OV.
    Journal: Immunol Lett; 1987 May; 15(1):33-9. PubMed ID: 3111988.
    Abstract:
    The investigation of 750 B-lymphocyte hybridoma clones obtained by fusion of mouse myeloma and newborn heterozygous Igk-1a/Igk-1b rat splenocytes has revealed that 9.8% of Ig kappa-chain loci are rearranged productively. Seventeen hybridomas secrete kappa-chains of both allelic variants. The analysis of IgM molecules of 9 such clones demonstrated that in 6 cases only one L-chain allotype, either 1a or 1b, is present in IgM. Thus for the first time the high frequency of selective association of H and L chains in Ig-producing cells was shown. Evidently this selectivity may function as one of allelic exclusion mechanisms at the Ig assembly stage.
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