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  • Title: Guanine nucleotides stimulate arachidonic acid release by phospholipase A2 in saponin-permeabilized human platelets.
    Author: Nakashima S, Tohmatsu T, Hattori H, Suganuma A, Nozawa Y.
    Journal: J Biochem; 1987 Apr; 101(4):1055-8. PubMed ID: 3112131.
    Abstract:
    GTP or GTP gamma S alone caused low but significant liberation of arachidonic acid in saponin-permeabilized human platelets but not in intact platelets. GTP or GTP gamma S also enhanced thrombin-induced [3H]arachidonic acid release in permeabilized platelets. Inhibitors of the phospholipase C (neomycin)/diacylglycerol lipase (RHC 80267) pathway for arachidonate liberation did not reduce the [3H]arachidonic acid release. The loss of [3H]arachidonate radioactivity from phosphatidylcholine was almost equivalent to the increase in released [3H]arachidonic acid, suggesting the hydrolysis of phosphatidylcholine by phospholipase A2. The effect of GTP gamma S was greater at lower Ca2+ concentrations. These data indicate that the release of arachidonic acid by phospholipase A2 in saponin-treated platelets may be linked to a GTP-binding protein.
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