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  • Title: Fabrication of a novel biosensor for biosensing of bisphenol A and detection of its damage to DNA.
    Author: Jalalvand AR, Haseli A, Farzadfar F, Goicoechea HC.
    Journal: Talanta; 2019 Aug 15; 201():350-357. PubMed ID: 31122434.
    Abstract:
    In this work, a novel electrochemical biosensor has been fabricated based on step-by-step modification of a glassy carbon electrode (GCE) with methylene blue (MB)-DNA/multiwalled carbon nanotubes (MWCNTs)-chitosan (CS)/palladium nanoparticles (Pd NPs)/fullerene C60 (C60) for voltammetric and impedimetric detection of DNA damage induced by bisphenol A (BPA). Modifications applied to the GCE were characterized by cyclic voltammetry (CV), differential pulse voltammetry (DPV), electrochemical impedance spectroscopy (EIS) and scanning electron microscopy. The EIS and DPV responses of the biosensor were increased and decreased, respectively, by the DNA damage induced by BPA which led us to develop novel systems for detection of DNA damage. Our records confirmed that the biosensor was able to rapid and sensitive detection of DNA damage induced by BPA. Finally, according to the developed systems for detection of DNA damage, we have developed voltammetric and impedimetric methods for determination of BPA.
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