These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Prostaglandin production by the guinea-pig endometrium: is calcium necessary?
    Author: Riley SC, Poyser NL.
    Journal: J Endocrinol; 1987 Jun; 113(3):463-71. PubMed ID: 3114408.
    Abstract:
    The output of prostaglandin (PG) F2 alpha from guinea-pig endometrium obtained on day 15 of the oestrous cycle and maintained in tissue culture was significantly (P less than 0.05) reduced by the use of Ca2+-depleted medium, EGTA (a Ca2+ chelator), 8-(N,N-diethyl-amino)octyl-3,4,5-trimethoxybenzoate hydrochloride (TMB-8; an intracellular Ca2+ antagonist), trifluoperazine (TFP) and N-(6-aminohexyl)-5-chloro-1-naphthalenesulphonamide (W-7; both calmodulin antagonists). Nifedipine inhibited PGF2 alpha output at a concentration (100 mumol/l) much greater than that usually required to block Ca2+ channels. Verapamil had a small but significant (P less than 0.05) inhibitory effect on PGF2 alpha output at 10-100 mumol/l. The outputs of PGE2 and, to a lesser extent, 6-keto-PGF1 alpha (the hydrated product of PGI2) were also reduced by using Ca2+-depleted medium. EGTA reduced the outputs of PGE2 and 6-keto-PGF1 alpha on day 1 of culture, but stimulated 6-keto-PGF1 alpha output on day 3 of culture. The outputs of PGE2 and 6-keto-PGF1 alpha were increased by TMB-8 (100 mumol/l) on day 3 of culture and by TFP and, to a smaller extent, by W-7 on all 3 days of culture. Nifedipine (100 mumol/l by not 1 or 10 mumol/l) reduced the outputs of PGE2 and 6-keto-PGF1 alpha on all 3 days of culture, whereas verapamil (100 mumol/l but not 1 or 10 mumol/l) increased the outputs of these two prostaglandins on days 2 and 3 of culture.(ABSTRACT TRUNCATED AT 250 WORDS)
    [Abstract] [Full Text] [Related] [New Search]