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Title: Identity of the V kappa 10-Ars-A gene segments of the A/J and BALB/c strains. Author: Meek K, Sanz I, Rathbun G, Nisonoff A, Capra JD. Journal: Proc Natl Acad Sci U S A; 1987 Sep; 84(17):6244-8. PubMed ID: 3114744. Abstract: To characterize the light chain gene segments involved in the murine immune response to keyhole limpet hemocyanin p-azophenylarsonate (Ars), we have determined the amino acid and/or nucleotide sequences of several anti-arsonate antibodies of the Ars-A family in the A/J, C.AL-20, and BALB/c strains. These structures have been compared to certain BALB/c anti-phenyloxazolone and anti-levan antibodies previously sequenced and to the A/J V kappa 10-Ars-A genomic sequence (where V kappa = kappa chain variable). These primary structural studies were complemented by Southern filter hybridization analyses utilizing V kappa and kappa chain joining (J kappa) molecular probes. We found a surprising uniformity of structure among these antibody light chains derived from different murine strains. Thus, in contrast to the heavy chain variable (Vh) regions of the Ars-A antibody family where the BALB/c strain lacks the VH gene segment utilized in the A/J Ars-A response, the light chain variable region gene segments at the V kappa 10-Ars-A locus appear to be identical between the two strains.[Abstract] [Full Text] [Related] [New Search]