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  • Title: Purification of a hemorrhagic factor (LHF-I) from the venom of the bushmaster snake, Lachesis muta muta.
    Author: Sánchez EF, Magalhães A, Diniz CR.
    Journal: Toxicon; 1987; 25(6):611-9. PubMed ID: 3114915.
    Abstract:
    Hemorrhagic factor (LHF-I), was isolated from Lachesis muta muta venom by a five-step procedure. Homogeneity was demonstrated by the presence of a single band on polyacrylamide gel electrophoresis, immunoelectrophoresis and immunodiffusion. LHF-I is a glycoprotein with molecular weight of approximately 100,000 as determined by sodium dodecyl sulfate polyacrylamide-gel electrophoresis. Caseinolytic activity was associated with the hemorrhagic activity throughout the purification procedure.
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