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  • Title: Diagnostic evaluation of the amastin protein from Leishmania infantum in canine and human visceral leishmaniasis and immunogenicity in human cells derived from patients and healthy controls.
    Author: Vale DL, Dias DS, Machado AS, Ribeiro PAF, Lage DP, Costa LE, Steiner BT, Tavares GSV, Ramos FF, Martínez-Rodrigo A, Chávez-Fumagalli MA, Caligiorne RB, de Magalhães-Soares DF, Silveira JAG, Machado-de-Ávila RA, Teixeira AL, Coelho EAF.
    Journal: Diagn Microbiol Infect Dis; 2019 Oct; 95(2):134-143. PubMed ID: 31155395.
    Abstract:
    The diagnosis of visceral leishmaniasis (VL) presents problems due to the toxicity and/or high cost of drugs. In addition, no vaccine exists to protect against human disease. In this study, the antigenicity and immunogenicity of amastin protein were evaluated in L. infantum-infected dogs and humans. For the diagnosis, besides the recombinant protein, 1 linear B-cell epitope was synthetized and evaluated in serological assays. Results showed high sensitivity and specificity values to detect the disease when both antigens were employed against a canine and human serological panel. By contrast, when using rA2 and a soluble Leishmania antigenic preparation, sensitivity and specificity values proved to be lower. A preliminary immunogenicity study showed that the amastin protein induced high IFN-γ and low IL-10 production in stimulated PBMC derived from treated VL patients and healthy subjects, thus suggesting a potential use of this protein as an immunogen to protect against human disease.
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