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  • Title: Influence of the culture medium on the production of iturin A by Bacillus subtilis.
    Author: Besson F, Chevanet C, Michel G.
    Journal: J Gen Microbiol; 1987 Mar; 133(3):767-72. PubMed ID: 3116164.
    Abstract:
    The production of iturin A by Bacillus subtilis was studied with respect to the composition of the culture medium. Increasing phosphate concentrations did not modify the antibiotic yield. Fructose, sucrose and mannitol were better carbon sources than glucose for antibiotic production. The nature of the nitrogen source was an important factor in the production of antibiotic. Among the amino acids which are components of iturin A, L-asparagine was the best substrate for the biosynthesis of iturin A; L-glutamine and L-serine were rather poor substrates while L-proline and D-tyrosine gave no antibiotic. Ammonium salts permitted good synthesis of antibiotic but the addition of calcium ions to the culture medium inhibited the excretion of antibiotic from the cells.
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