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  • Title: [Sorption immobilization of alpha-amylase from Bacillus subtilis using meshed carboxyl polyelectrolytes].
    Author: Glazova NV, Tishchenko GA, Murav'eva TD, Potekhina TS, El'kin GE.
    Journal: Prikl Biokhim Mikrobiol; 1987; 23(4):542-6. PubMed ID: 3116527.
    Abstract:
    Immobilization of alpha-amylase from Bacillus subtilis by adsorption on carboxyl polyelectrolytes, copolymers of methacrylic acid and ethylene glycol dimethacrylate, was being investigated depending on the structure of the polymeric matrix. It is demonstrated that cation exchange resins can be successfully used for reversible sorption and immobilization of alpha-amylase. It was found that the more heterogeneous the structure of the polymeric carrier, the higher is reversibility of the enzyme adsorption. The isothermic process of Bac. subtilis alpha-amylase adsorption on copolymers of different structures was studied as well. The equilibrium of the sorption system was proved to be true.
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