These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.
Pubmed for Handhelds
PUBMED FOR HANDHELDS
Search MEDLINE/PubMed
Title: Mouse UDP glucuronosyltransferase. cDNA and complete amino acid sequence and regulation. Author: Kimura T, Owens IS. Journal: Eur J Biochem; 1987 Nov 02; 168(3):515-21. PubMed ID: 3117546. Abstract: A cDNA clone (UDPGTm-1) encoding a mouse UDP glucuronosyltransferase (transferase) was isolated from pBR322 and lambda gt11 libraries by hybridization to a rat transferase clone. This cDNA is 1860 bp long and 65-87% similar at both the nucleotide and the deduced amino acid sequence levels to three different rat transferase clones [Mackenzie, P.I. et al. (1984) J. Biol. Chem. 259, 12153-12160; Mackenzie, P.I. (1986) J. Biol. Chem. 261, 6119-6125]. UDPGTm-1, like the rat transferase clones already described, contains an open reading frame of 1590 bp encoding 530 amino acids (unmodified Mr = 60,856), an N-terminus membrane-insertion signal sequence, a carboxy-terminus hydrophobic putative membrane-spanning region, and potential asparagine-linked glycosylation sites (residues 316 and 483). The cDNA contains two poly(A) addition consensus sequences at positions 1695-1837. UDPGTm-1 is complementary to a 2200-base mRNA and also cross-hybridizes to a 2000-base mRNA species due to sequence homology in the 5' region of the clone. Both the 2200-base and the 2000-base mRNA are induced approximately 2.5-fold by the hypolipidemic agent clofibrate, and also by phenobarbital and benzo[a]pyrene. A new and more potent hypolipidemic agent, perfluorooctanoic acid, is also shown to induce both mRNA species. Each of these compounds induces bilirubin transferase activity in a manner parallel to the effects on mRNA, i.e. perfluorooctanoic acid being the most effective, followed by phenobarbital, benzo[a]pyrene, and clofibrate. Southern blot hybridization of UDPGTm-1 to mouse genomic DNA showed sequence homology to a total DNA size of 40-50 kb. These data indicate that UDPGTm-1 is a member of a new subfamily of transferases in mouse with patterns of regulation of their mRNAs similar to that seen for bilirubin transferase activity.[Abstract] [Full Text] [Related] [New Search]