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  • Title: Epitope approach in molecular imprinting of antibodies.
    Author: Moczko E, Guerreiro A, Cáceres C, Piletska E, Sellergren B, Piletsky SA.
    Journal: J Chromatogr B Analyt Technol Biomed Life Sci; 2019 Aug 15; 1124():1-6. PubMed ID: 31176265.
    Abstract:
    Herein an approach to prepare molecularly imprinted polymer nanoparticles (nanoMIPs) with specific binding affinity for antibodies is reported. The process relied on the covalent immobilization of the template (whole immunoglobulin G (IgG), Fc domain of human IgG and peptide epitope) onto the surface of a solid support, polymerization and affinity separation of nanoMIPs. The binding between nanoMIPs and their corresponding templates was analyzed and evaluated as being in sub-nanomolar and nano-molar range. The nanoMIPs prepared for Fc domain and epitope demonstrated a specific recognition of both human and goat IgGs, therefore they could be considered as a synthetic analogue of protein A and benefit from its intrinsic stability, short time and low cost of preparation.
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