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  • Title: A specific AIE and ESIPT fluorescent probe for peroxynitrite detection and imaging in living cells.
    Author: Shen Y, Li M, Yang M, Zhang Y, Li H, Zhang X.
    Journal: Spectrochim Acta A Mol Biomol Spectrosc; 2019 Nov 05; 222():117230. PubMed ID: 31177001.
    Abstract:
    Developing specific and sensitive method for endogenous peroxynitrite (ONOO-) in living systems is valuable to understand its various pathological events. In this work, a simple and novel fluorescent probe (HPP) based on aggregation induced emission (AIE) as well as excited state intramolecular proton transfer (ESIPT) processes for endogenous ONOO- detection was constructed containing a diphenylphosphinate as the reactive site and a salicylaldehyde azine as the fluorophore. The probe showed specific fluorescence turn-on response toward ONOO- owing to the diphenylphosphinate group of HPP reacted with ONOO-, releasing the salicylaldehyde azine with both AIE and ESIPT characteristics. In addition, the probe exhibited a large Stokes shift, an excellent light-up ratio, high selectivity and excellent sensitivity with a low detection limit of 8 × 10-8 M for endogenous ONOO-. Moreover, the probe could be applied to bioimaging of endogenous ONOO- in live cell, which demonstrated the probe can be used as effective tool for investigation of ONOO- in biological systems.
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