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  • Title: The combined use of immunohistochemistry and intracellular staining with horseradish peroxidase for light and electron microscopic studies of transmitter-identified inputs to functionally characterized neurons.
    Author: Ulfhake B, Cullheim S, Hökfelt T, Visser TJ.
    Journal: Brain Res; 1987 Sep 01; 419(1-2):387-91. PubMed ID: 3119147.
    Abstract:
    Physiologically identified triceps surae alpha motoneurons in the cat were stained intracellularly with horseradish peroxidase (HRP). After fixation with 2% glutaraldehyde and treatment with sodium borohydride, spinal cord sections were incubated with rabbit antiserum against thyrotropin-releasing hormone (TRH) and rabbit peroxidase-antiperoxidase complex. Light microscopically detected close contacts between immunoreactive nerve terminals and intracellularly HRP-stained profiles were studied under the electron microscope. In this way, synaptic contacts between TRH-immunoreactive boutons and functionally characterized alpha motoneurons could be demonstrated.
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