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  • Title: Effect of dietary energy concentration on the response of laying hens to amino acids.
    Author: Gous RM, Griessel M, Morris TR.
    Journal: Br Poult Sci; 1987 Sep; 28(3):427-36. PubMed ID: 3119163.
    Abstract:
    1. A hypothesis, that the optimum amino acid concentration in the diet is not directly proportional to the dietary energy concentration, but changes in inverse proportion to the change in food intake resulting from a change in energy concentration, was tested in three experiments. 2. Response experiments involving the amino acids methionine, lysine and isoleucine were conducted, in each case at three dietary energy concentrations, using a diet dilution and blending technique, thereby ensuring a constant ratio between background amino acids and the first-limiting amino acid in all diets, and also keeping the ratio of amino acids to energy constant as energy varied. 3. A common response curve relating egg output (g/bird d) to amino acid intake (mg/bird d) for each amino acid, fitted by means of the Reading Model, adequately described the response at each of the dietary energy contents. This implies that energy does not influence egg output directly, but only indirectly through its effect on food intake and hence on amino acid intake. 4. Both amino acid and energy concentration significantly influenced food intake. Energy intake was not constant over all dietary energy concentrations, being lower at low energy levels and higher at high energy concentrations. 5. It is concluded that amino acid requirements should not be stated either as percentages or as ratios with energy. Optimum amino acid intakes and energy concentrations should be calculated; the expected food intake should then be predicted, after which the appropriate concentration of nutrients in the diet can be determined.
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