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  • Title: [Integration and expression of the human dihydrofolate reductase gene in the Bacillus subtilis chromosome].
    Author: Poluéktova EU, Savchenko GV, Nezametdinova VZ, Khasanov FK, Prozorov AA.
    Journal: Genetika; 1987 Oct; 23(10):1839-46. PubMed ID: 3121440.
    Abstract:
    Integration of functionally active human dihydrofolate reductase (hDHFR) gene into the Bacillus subtilis chromosome was performed. The clones obtained contained 1 to 7 copies of hDHFR gene per chromosome equivalent and were resistant to trimethoprim. In cell lysates of such clones a protein with the molecular mass of hDHFR was detected. The hDHFR gene was stably maintained in all clones having this gene integrated into the bacterial chromosome, when grown under non-selective conditions.
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