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  • Title: [Effect of zoladronate on the proliferation and osteogenic differentiation of rat bone mesenchymal stem cells].
    Author: Li JH, Su ZF, Bai X, Yuan H, Li JY.
    Journal: Hua Xi Kou Qiang Yi Xue Za Zhi; 2019 Jun 01; 37(3):242-247. PubMed ID: 31218855.
    Abstract:
    OBJECTIVE: To evaluate the effect of zoledronate acid (ZA) on the proliferation and osteogenic differentiation of rat mesenchymal stem cells (BMSCs). METHODS: The BMSCs isolated from the SD rats were cultured with different concentrations of ZA (1, 5, 10, and 20 μmol·L), and the contro1 group received the same volume of culture medium but without ZA. Cell counting kit-8 was used to detect proliferation activity in each group. Alkaline phosphatase (ALP) staining and alizarin red staining were used to detect the osteogenic differentiation ability in each group. The gene expression levels of ALP, bone morphogenetic protein-2 (BMP-2), typeⅠcollagenase (COL-Ⅰ), runt-related transcription factor-2 (Runx-2), zinc finger structure transcription factor (Osx), osteocalcin (OCN), and osteopontin (OPN) were evaluated by real-time quantitative polymerase chain reaction (qRT-PCR). RESULTS: Zoledronate at 1 μmol·L⁻¹ concentration had no effect on the proliferation and osteogenic differentiation of BMSCs. No significant difference was observed between this group and the control group (P>0.05). When the ZA concentration was more than 1 μmol·L⁻¹, ZA inhibited the proliferation and osteogenic differentiation of BMSCs, and the effect was concentration dependent. The difference between each group and the control group was statistically significant (P<0.05). At ZA concentration of 5 μmol·L⁻¹, ZA enhanced the expression of ALP, BMP-2, COL-Ⅰ, Runx-2, Osx, OCN, and OPN (P<0.05). However, at ZA concentration of more than 5 μmol·L⁻¹, the expression levels of osteogenicrelated genes in each group was lower than those of the control group (P<0.05). CONCLUSIONS: Low ZA concentration has no effect on the proliferation and osteogenic differentiation of BMSCs. ZA at 5 μmol·L⁻¹ concentration inhibits the proliferation but promotes the osteogenic differentiation of BMSCs. High ZA concentration inhibits the proliferation and osteogenic differentiation of BMSCs. 目的 研究唑来膦酸对大鼠骨髓间充质干细胞（BMSCs）增殖及成骨分化的作用。方法 取大鼠BMSCs体外原代培养，使用含不同浓度（1、5、10、20 μmol·L⁻¹）唑来膦酸的成骨诱导培养基干预细胞作为实验组，不含唑来膦酸的培养基干预细胞作为对照组。CCK-8检测各组细胞增殖活性；茜素红和碱性磷酸酶染色检测各组细胞成骨分化能力；实时荧光定量聚合酶链反应（qRT-PCR）检测各组细胞碱性磷酸酶（ALP）、骨形态发生蛋白2（BMP-
2）、Ⅰ型胶原酶（COL-Ⅰ）、Runt相关转录因子2（Runx-2）、锌指结构转录因子（Osx）、骨钙蛋白（OCN）、骨桥蛋白（OPN）的mRNA表达量。结果 1 μmol·L⁻¹唑来膦酸对BMSCs的增殖、成骨分化无影响，与对照组比较差异无统计学意义（P>0.05）。浓度大于1 μmol·L⁻¹唑来膦酸抑制BMSCs的增殖和成骨分化，且抑制作用呈浓度依赖性，与对照组比较差异有统计学意义（P<0.05）。当唑来膦酸浓度为5 μmol·L⁻¹时，ALP、BMP-2、COL-Ⅰ等成骨相关基因的表达量高于对照组（P<0.05），而当唑来膦酸浓度大于5 μmol·L⁻¹时，成骨相关基因的表达量低于对照组（P<0.05）。结论 低浓度唑来膦酸对BMSCs增殖和成骨分化无影响，5 μmol·L⁻¹唑来膦酸抑制BMSCs增殖但促进其成骨分化，高浓度唑来膦酸抑制BMSCs的增殖和成骨分化。.
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