These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Random Mutagenesis of Virus Gene for the Experimental Evaluation of the Durability of NB-LRR Class Plant Virus Resistance Gene.
    Author: Idehara K, Tomita R, Sekine KT, Nishiguchi M, Kobayashi K.
    Journal: Methods Mol Biol; 2019; 2028():97-113. PubMed ID: 31228110.
    Abstract:
    NB-LRR class plant virus resistance gene is a one of the key players that shape the plant-virus interaction. Evolutionary arms race between plants and viruses often results in the breakdown of virus resistance in plants, which leads to a disastrous outcome in agricultural production. Although studies have analyzed the nature of plant virus resistance breakdown, it is still difficult to foresee the breakdown of a given virus resistance gene. In this chapter, we provide a protocol for evaluating the durability of plant virus resistance gene, which comprises the random mutagenesis of a virus gene, the introduction of the mutagenized gene into a virus context with highly efficient inoculation system, and the efficient screening of virus mutants that can overcome or escape a virus resistance.
    [Abstract] [Full Text] [Related] [New Search]