These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: A semiconductor quantum dot-based ratiometric electrochemical aptasensor for the selective and reliable determination of aflatoxin B1.
    Author: Wang C, Qian J, An K, Lu X, Huang X.
    Journal: Analyst; 2019 Aug 05; 144(16):4772-4780. PubMed ID: 31268094.
    Abstract:
    In recent years, a ratiometric electrochemical method has been investigated due to its ability to effectively reduce the background electrical signals via the introduction of an internal calibration mechanism, which has great practical significance in the detection of mycotoxins in foods. Herein, we report a ratiometric electrochemical aptasensor based on two semiconductor quantum dots (i.e. CdTe and PbS QDs) for the detection of aflatoxin B1 (AFB1). The aptasensor was fabricated by immobilizing PbS QD-coated silica hybrid spheres (SiO2@PbS) onto CdTe QD-modified Fe3O4@SiO2 (Fe3O4@SiO2/CdTe) surface through biorecognition between the aptamer and complementary DNAs, where PbS QDs acted as external signal labels and CdTe QDs acted as internal reference labels. In the presence of AFB1, the aptamer connected to SiO2@PbS preferred to form an aptamer/AFB1 complex, which brought about the separation of SiO2@PbS linked with the CdTe QDs; with the addition of more AFB1 to the solution, the amount of SiO2@PbS present on the Fe3O4@SiO2/CdTe surface reduced. After several steps of endonuclease cleavage, magnetic separation, and dissolution with acid, the square wave voltammetry signals of Pb2+ and Cd2+ maintained an inverse relationship with the target content based on the SWV stripping measurements; the proposed method had the wide linear range of 5 pg mL-1-50 ng mL-1 and the determination limit of 4.5 pg mL-1 (S/N = 3) and was applied for the detection of AFB1 in peanuts. The proposed aptasensor has an important practical significance for the development of food safety.
    [Abstract] [Full Text] [Related] [New Search]