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  • Title: Ca2+ ionophore A23187-induced rise in cytoplasmic free calcium and iodide discharge in porcine thyroid cells: measurement of cytoplasmic free calcium by aequorin.
    Author: Takasu N, Murakami M, Nagasawa Y, Yamada T, Shimizu Y, Kojima I, Ogata E.
    Journal: J Endocrinol; 1987 Dec; 115(3):477-80. PubMed ID: 3127512.
    Abstract:
    The cytoplasmic concentration of free calcium was measured using aequorin, a calcium-sensitive photoprotein. The Ca2+ ionophore A23187 induced a rise in cytoplasmic free calcium and iodide discharge in cultured porcine thyroid cells. The minimum dose of A23187 effecting an increase in cytoplasmic free calcium induced iodide discharge. The A23187-induced rise in cytoplasmic free calcium was followed by iodide discharge. The results indicate that A23187-induced iodide discharge is mediated by a rise in the cytoplasmic concentration of free calcium.
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