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  • Title: An ELISA using an SDS extract of Brucella abortus strain 99 as antigen to detect B. abortus antibodies in cattle sera.
    Author: Williamson CC, Oberem PT, Poerstamper C, De Waal DT, Matthee O, Brett OL.
    Journal: Onderstepoort J Vet Res; 1988 Mar; 55(1):1-3. PubMed ID: 3127768.
    Abstract:
    An ELISA was developed using an SDS extract of Brucella abortus as antigen to detect antibodies in cattle sera. The antigen was stable at 4 degrees C for at least 4 years and although it gave optimal results at a 1/4,000 dilution it could detect reactors at a 1/32,000 dilution. Based on comparative CF tests on 430 sera from negative herds and 187 sera from positive herds a reading of 0,07 or less using a 492 nm filter could be considered a negative reaction. The ELISA could be a useful test to supplement the CF test but further evaluation is still required.
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