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  • Title: [Isolation and identification of endophytic fungi producing harpagoside and harpagide from Scrophularia ningpoensis].
    Author: Shen ZY, Zhu B, Zhang QL, Qin LP.
    Journal: Zhongguo Zhong Yao Za Zhi; 2019 May; 44(10):2046-2050. PubMed ID: 31355559.
    Abstract:
    The endophytic fungi from root,main stem,branch and leaf of Scrophularia ningpoensis were isolated from Zhejiang,whether these strains could yield harpagide or harpagoside were tested by HPLC and LC-MS. According to the morphological characteristic and the similarity of the nucleotide sequence of internal transcribed spacer( ITS) between r DNAs,the strains producing harpagide or harpagoside were identified. The results showed that 210 strains were isolated from the samples,which were classified into 9 orders,13 families and 17 genera by morphological study. Harpagide was detected in endogenous fungi ZJ17 and harpagoside was detected in endogenous fungi ZJ25 by HPLC coupled with LC-MS. ZJ17 was identified as Alternaria alternate and ZJ25 was identified as A.gaisen by its morphology and authenticated by ITS( ITS4 and ITS5 regions and the intervening 5. 8 S rDNA region).
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