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  • Title: Colorimetric determination of xanthine in urine based on peroxidase-like activity of WO3 nanosheets.
    Author: Li Z, Liu X, Liang XH, Zhong J, Guo L, Fu F.
    Journal: Talanta; 2019 Nov 01; 204():278-284. PubMed ID: 31357294.
    Abstract:
    Two-dimensional WO3 nanosheets were prepared by ultrasonic exfoliation of bulk WO3·2H2O in water and characterized by transmission electron microscopy, atomic force microscopy, X-ray diffraction, X-ray photoelectron spectroscopy, Raman, dynamic light scattering. The nanosheets were discovered to possess the peroxidase-like catalytic activity, which can catalyze the oxidation of 3, 3', 5, 5'-tetramethylbenzidine by H2O2. The catalytic mechanism was also investigated by the scavenger experiments. Taking advantage of the peroxidase-like activity of WO3 nanosheets, a facile colorimetric method for xanthine was developed by combining the oxidation reaction of xanthine catalyzed by xanthine oxidase. The linear range for xanthine was ranged from 25 to 200 μmol L-1. The limit of detection for xanthine was 1.24 μmol L-1. The colorimetric method was applied to determine xanthine in urine samples.
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