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  • Title: [Expression in Escherichia coli of the human leukocyte interferon alpha2 gene fused with N-terminal fragment of beta-galactosidase].
    Author: Petrenko VA, Tat'kov SI, Semenova LN, Il'ichev AA, Pozdniakov PI.
    Journal: Mol Gen Mikrobiol Virusol; 1988 Apr; (4):37-41. PubMed ID: 3136326.
    Abstract:
    Genes for leucocyte interferon and alpha-donor of galactosidase were fused by deletion mutagenesis or by site-directed mutagenesis. In both cases the fused protein was expressed. The protein having an antiviral activity of leucocyte interferon was easily detected in bacteria and solutions by the reaction of beta-galactosidase alpha-complementation and retained the antigenic determinants of interferon and beta-galactosidase. The use of fused proteins for optimization of gene expression and for the analysis of interferon structure-function relationship is discussed.
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