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  • Title: [Intracellular lumen in cultured hepatocytes of rats. Study of an original cytoplasmic infrastructure].
    Author: Remy L, Reynier MO, Hashieh IA, Gerolami A.
    Journal: C R Acad Sci III; 1988; 307(2):63-8. PubMed ID: 3138006.
    Abstract:
    A few hours after plating, isolated rat hepatocytes reassociate into clusters and differentiate intercellular cavities bordered by junctional complexes. These structures show a great resemblance to bile canaliculi seen in vivo. Intracellular lumens surrounded by microvilli are observed in the cytoplasm of some cultured hepatocytes. The formation of these structures, which contain an osmiophilic substance, probably results from modifications in the functioning of the secretory apparatus. It can be speculated that these intracellular lumens may contribute to the formation of new canaliculi differentiated between reassociated cells.
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