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  • Title: Hydroxyprolyl3-bradykinin in high molecular mass kininogen. Presence in human and monkey kininogens, but not in kininogens from bovine, rat, rabbit, guinea pig and mouse plasmas.
    Author: Enjyoji K, Kato H.
    Journal: FEBS Lett; 1988 Sep 26; 238(1):1-4. PubMed ID: 3139453.
    Abstract:
    The contents of hydroxyprolyl3-bradykinin in high molecular mass (HMM) kininogens from human and animal plasmas were examined by reversed-phase HPLC following their proteolytic scission by bovine plasma kallikrein. The relative contents of hydroxyprolyl3-bradykinin in kinins from HMM kininogens from pooled plasmas of human and monkey origin were 33 and 73%, respectively. On the other hand, hydroxyprolyl3-bradykinin could not be detected in HMM kininogen preparations from bovine, rat, guinea pig, rabbit and mouse plasmas. Hydroxyproline in hydroxyprolyl3-bradykinin was assigned as trans-4-hydroxyproline by comparison of the retention times on reversed-phase HPLC with isomers of hydroxyproline after derivatization with 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole chloride.
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