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  • Title: Basal and amiloride-induced short-circuit current across isolated toad skin (Bufo arenarum).
    Author: Soria MO, Berman DM, Coviello A.
    Journal: Gen Physiol Biophys; 1988 Aug; 7(4):395-9. PubMed ID: 3141246.
    Abstract:
    We have previously demonstrated that amiloride (amil) addition to the isolated ventral pelvic (VPel) skin of Bufo arenarum toad induces negative short-circuit current values, which are equivalent to the isotopically measured net chloride transport. In the present work, we found that exposure of various regions of toad skin to amil yielded different values of short-circuit current (aSCC): negative aSCC was found in the VPel and ventral pectoral skin, while those of the dorsal one were not different from zero. The distinct values of aSCC found show a regional difference in the active chloride absorption, probably related to postural adaptations. A possible role of this adaptation would be related to chloride participation in the saline balance of the animals, or the maintenance of epithelial integrity.
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