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Title: [Adenanthin Induces Differentiation of Acute Promyelocytic Leukemia Cells by Targeting Peroxiredoxin III].
Author: Mo YX, Liu DL, Gao SL, Yin XC.
Journal: Zhongguo Shi Yan Xue Ye Xue Za Zhi; 2019 Aug; 27(4):1083-1087. PubMed ID: 31418361.
Abstract:
OBJECTIVE: To investigate the differentiation of acute promyelocytic leukemia (APL) cells induced by adenosine targeting Prx III. METHODS: HL-60 cells were divided into four groups: control group, all-trans retinoic acid (ATRA) group, adenanthin group and ATRA+adenanthin group. Cell morphologic changes were observed under optical microscope. The influence of adenanthin on the differentiation of HL-60 was observed by nitro blue tetrazolium chloride (NBT) test. Cell surface differentiation antigens CD11b expression was measured by flow cytometry. The protein expression of Prx III was detected by immunohistochemical assay. RESULTS: Adenanthin could induce the differentiation of HL-60 cells; the NBT reduction positive rate in ATRA+adenanthin group was significantly higher than that in ATRA group and adenanthin group (P＜0.05). The percentage of CD11b positive cells in ATRA+adenanthin group (43.62％±1.38％) was higher than that in adenanthin group (28.15％±1.78％), ATRA group (36.72％±1.33％) and control group (7.99％±1.78％) (P＜0. 05). The content of Prx Ⅲ protein in adenanthin group was significantly higher than that in control group and ATRA group (P＜0.05). CONCLUSION: Adenanthin and ATRA have a synergistic effect on the differentiation and maturation of HL-60 cells, and its mechanism may be related with regulation of Prx III expression. 题目: 腺花素通过靶向 Prx III 诱导急性早幼粒白血病细胞分化的研究. 目的: 探讨腺花素（Adenanthin）靶向Prx III 诱导急性早幼粒白血病（acute promyelocyticleukemia，APL）细胞分化. 方法: 以HL-60细胞株作为研究对象，取对数生长期细胞，分为对照、全反式维甲酸（ATRA）、Adenanthin和ATRA+Adenanthin，共4组。观察各组细胞形态的变化；用NBT还原实验分析细胞分化能力的变化；流式细胞术检测细胞表面分化抗原CD11b的表达变化；采用Western blot 检测Prx III表达的变化. 结果: Adenanthin可诱导HL-60细胞分化；ATRA+Adenanthin组NBT还原阳性率明显高于ATRA组和Adenanthin组（P＜0.05）；ATRA+Adenanthin组CD11b阳性细胞百分率（43.62％±1.38％）均明显高于Adenanthin组（28.15％±1.78％）、ATRA组（36.72％±1.33％）及空白对照组（7.99％±1.78％）（P＜0.05）；Adenanthin组Prx Ⅲ蛋白水平明显高于空白对照组及ATRA组（P＜0.05）. 结论: 腺花素能协同ATRA使HL-60细胞分化成熟，其作用机制可能与调控 Prx III的表达有关.

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