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Title: [Relationship of Peripheral Blood IL-37 Expression with T Lymphocytes Subsets and NK Cells in Patients with Primary Immune Thrombocytopenia].
Author: Chen Z, Qu W, Wang HQ, Xing LM, Wu YH, Liu ZY, Zhang Y, Liu H, Dong XF, Tao JL, Shao ZH.
Journal: Zhongguo Shi Yan Xue Ye Xue Za Zhi; 2019 Aug; 27(4):1201-1207. PubMed ID: 31418380.
Abstract:
OBJECTIVE: To study the correlation of IL-37 with T lymphocytes subsets and NK cells in ITP patients, and to explore its possible mechanisms involved in the pathogenesis of ITP. METHODS: Forty-five patients with newly diagnosed ITP(newly diagnosed group), 32 patients of complete remission (remission group) and 22 healthy persons(control group) were selected. The serum level of IL-37 in 3 groups was determined by enzyme linked immunosorbent assay (ELISA). The mRNA expression of IL-37, IL-17 and IL-18 in peripheral blood mononuclear cells（PBMNC） in 3 groups was measured by real-time fluorescence quantitative polymerase chain reaction (PCR). The number of IL-18Rα⁺CD4⁺ T cells and Tim-3⁺NK cells in the peripheral blood in 3 groups was detected by flow cytometry (FCM). RESULTS: The serum level of IL-37 in the peripheral blood of ITP patients in the newly diagnosed group was significantly higher than that in the control group and the remission group(P＜0.01) . The expression level of IL-37 in PBMNC of the ITP patients in newly diagnosed group was higher than that in the control group and the remission group(P＜0. 05). The expression level of IL-17 and IL-18 in PBMNC of the ITP patients in newly diagnosed group was higher than that in the control group and the remission group(P＜0. 01); the expression of IL-18Rα in CD4⁺ T cells in newly diagnosed group was significantly higher than that in both the control and the remission group(P＜0.01).The expression of Tim-3 in NK cells in ITP patients was significantly lower than that in the control group (P＜0. 01). In ITP patients, the serum IL-37 level and IL-18Rα⁺CD4⁺T cells ratio both negatively correlated with Plt count (r=-0.58, r=-0.48) moreo-ver the serum IL-37 level also negatively correlated with amount of CD4⁺ T cells and NK cells (r=-0.29, r=-0.28), but positively correlated with amount of CD8⁺ T cells (r=0.329). CONCLUSION: The IL-37 and its receptors may play an immunoregulatory role in CD4⁺ T cells and NK cells, the IL-37 may be a therapeutic target for ITP patients. 题目: 原发免疫性血小板减少症(ITP) 患者外周 IL-37的水平变化及其与T亚群、NK细胞的相关性研究. 目的: 分析ITP 患者外周血IL-37表达水平变化及其与T亚群、NK细胞数的相关性，探讨其参与疾病发病的可能机制. 方法: 选取初诊组ITP 患者45 例，完全缓解组ITP 患者32例及正常对照组22例。采用酶联免疫吸附试验法( ELISA) 测定3 组外周血血清中IL-37水平，应用实时荧光定量聚合酶链式反应(PCR) 测定3 组外周单个核细胞内IL-37、IL-18、IL-17 mRNA 相对表达水平,采用流式细胞术(FCM) 检测3 组IL-18Rα⁺CD4⁺ T细胞比例，2组Tim-3⁺ NK细胞比例. 结果: 初诊组ITP患者血清IL-37水平高于缓解组患者及正常对照组（P<0.01）；初诊组IL-37 mRNA表达水平高于缓解组及正常对照组（P<0.01），缓解组IL-37 mRNA表达水平高于正常对照组（P<0.05）。初诊组IL-18、IL-17 mRNA表达水平均明显高于缓解组及正常对照组（P<0.01）。流式细胞术检测表明，初诊组的IL-18Rα⁺CD4⁺ T细胞比例比缓解组和对照组增高，且差异具有统计学意义。初诊组的Tim-3⁺ NK 细胞比例比对照组低，且差异具有统计学意义（P<0.01）;在ITP患者中，血清IL-37水平和IL-18Rα⁺CD4⁺ T细胞比例均与血小板数量呈负相关(r=-0.58; r=-0.48)，与CD4⁺ T细胞数、NK细胞数呈负相关(r=-0.29; r=-0.28)，与CD8⁺T细胞量呈正相关（r=0.329）. 结论: IL-37及其受体可能在ITP患者的CD4⁺ T细胞和NK细胞发挥免疫调节作用，IL-37可以成为免疫性血小板患者的治疗靶标.

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