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  • Title: Kinetic characterization of the acyl-enzyme mechanism for beta-lactamase I.
    Author: Martin MT, Waley SG.
    Journal: Biochem J; 1988 Sep 15; 254(3):923-5. PubMed ID: 3143353.
    Abstract:
    beta-Lactamase I catalyses the hydrolysis of penicillins by an acyl-enzyme mechanism. A procedure was developed for determining the rate constants for the acylation and deacylation steps for the good substrates benzylpenicillin and phenoxymethylpenicillin; this depends on determining the fraction of enzyme that is present as acyl-enzyme in the steady state.
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