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  • Title: Production and partial characterization of elastase of Bacillus subtilis isolated from the cervices of human females.
    Author: Kaur M, Tripathi KK, Gupta M, Jain PK, Bansal MR, Gupta KG.
    Journal: Can J Microbiol; 1988 Jul; 34(7):855-9. PubMed ID: 3143474.
    Abstract:
    Conditions are described for the production of extracellular elastase by Bacillus subtilis. The yield of enzyme was maximum in shake-cultures grown in Syncase medium at 37 degrees C and was stable in culture supernatants. The enzyme, purified by ammonium sulphate precipitation and Sephadex G-75 gel filtration, showed a molecular weight of 25,000 and activity between pH 6.0 and 9.5, with an optimum of 9.0 in Tris-maleate buffer. Elastinolytic activity was maximum in glycine-NaOH buffer and minimum in phosphate buffer. Enzyme activity was adversely affected by temperature greater than or equal to 40 degrees C.
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